Abstract

Objective To ifivestigate hepatitis G virus (HGV) infection in hepatitis patients and the gene type of HGV in Guangzhou. Methods Reverse transcriptase-nested polymerase chain reaction (RTnested PCR) method using primers locating in noncoding region of HGV genome was used for detecting HGV RNA in sera of hepatitis patients and the amplified products were directly sequenced. Results 25 cases were HGV RNA positive in 251 sera of acute and chronic hepatitis(9.96% ) ,of which 4 cases were positive in 56 sera with hepatitis non A-E(7.14%),8 cases were positive in 77 sera with hepatitis B ( 10.39 % ), 13 cases were positive in 118 sera with hepatitis C ( 11.17 % ). The identity of nucleotide sequence was 98.0 % between 2 Guangzhou HGV isolates, both were 91.1% with American isolate, 81.7 % and 84.2 % respectively between 2 Guangzhou isolates and African isolate. Conclusion HGV infection exists in hepatitis patients, 2 Guangzhou HGV isolates may belong to the same gene type and both of them have a high homology with American HGV isolate.

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