Abstract
Objective To study HPeV from stool samples of children with acute gastroenteritis under 5 years old. Methods We conducted a real-time PCR to detect HPeV from stool samples and to amply VP1 sequence by nested RT-PCR to identify HPeV type. Results The results showed that 27 of 306 (8.82%) children with acute gastroenteritis were infected HPeV. 11 strains were typed. 9 strains HPeV1,both HPeV2 and HPeV4 was 1 strain. HPeV was mostly identified in autumn season with a peak in July.HPeV seemed relevant in children ＞ 2 years old. The range of nucleotide identity between all isolated strains with reference strains was 79%-92%. Conclusion Epidemiology characteristic of HPeV in Jilin was concordance with that of reports. HPeV3 wasnt detected. It's significant to conduct the large scale and longterm surveillance of HPeV. Key words: Parechovirus; Diarrhea
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