Abstract
Testosterone, the principal androgen of the higher vertebrates, has been demonstrated in only a few species of fish (see Bern & Chieffi, 1968). Although there is circumstantial evidence for the presence of a testicular steroidal androgen in the African cichlid fish of the genus Tilapia (Levy & Aronson, 1955; Marshall & Lofts, 1956; Yaron, 1966; Hyder, 1969), the nature of the androgen has remained unknown. The object of this study was to ascertain whether testosterone could be identified in the testes of Tilapia. Testes from ten sexually mature Tilapia leucosticta were collected for this study, the fish being freshly caught from spawning areas of Lake Naivasha. They were immediately weighed, measured and their testes removed and weighed. Each pair of gonads was minced and extracted with diethyl ether; the ether extract was dried and shipped to the National Cancer Institute, Bethesda, for analysis. Tritiated testosterone (approx. 10,000 d.p.m. = 0·06 ng.)
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