Abstract

In this study, the non-polar light petroleum ether and diethyl ether extracts of date palm (Phoenix dactylifera L.) Pollen were analyzed by Gas chromatography-mass spectroscopy (GC-MS) and found to be rich with lipids and steroids. Fatty acids include Palmitic acid 38.52% and Hydnocarpic acid 44.23% while α- Sitosterol was the most prevalent Phytosterol 14.40%. Un-saponifiable fraction and saponifiable fraction of Pet. ether and DEE extracts were screened for their in vitro antimicrobial activities against six bacterial species (Escherichia coli, Klebsiella species, Staphylococcus epidermidis, Bacillus cereus, Micrococcus luteus and Staphylococcus aureus). All fractions have good antibacterial activity against the six studied bacterial species. But it gave high antifungal activity as compared with Ketoconazole (standard) except with Un-sap. fraction of Pet. ether extract with Candida albicans and Aspergillus niger it gave antifungal activity with Un-sap. fraction of Pet. ether extract only. Finally two extracts were tested against three human cell lines HELA, MCF7 and CACO. The results showed those extracts had activity against all cell lines tested.

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