Abstract

Detection and differentiation of sheeppox virus and goatpox virus from clinical samples using 30 kDa RNA polymerase subunit (RPO30) gene based PCR

Highlights

  • There are many diseases affecting sheep and goats

  • The current study demonstrated that RPO30 gene based PCR assay could be used for molecular epidemiology of capripox virus infection and differentiation of causative agent viz., sheep pox virus and goatpox virus

  • The present study describes the use of RPO30 gene based PCR for genotype identification of capripox viruses from clinical samples of pox disease outbreaks

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Summary

Introduction

Sheeppox and goatpox are the two major diseases which are caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively. These viruses belong to the genus Capripoxvirus of the family Poxviridae [1]. The genus Capripoxvirus (CaPV) includes lumpy skin disease virus (LSDV), which causes lumpy skin disease/nodular dermatitis in cattle. Sheeppox and goatpox exhibit similar clinical signs characterized by pyrexia and pock lesions in the skin and lungs. Sheeppox and goatpox are endemic in Africa, India, North Equator, Middle East countries including Egypt, Turkey, Iraq, Iran, and Afghanistan, and India [1, 3]. In India, sheeppox and goatpox are have been reported from almost all the states [4,5,6]

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