Abstract
A systematic detection and determination method for organic impurities in 7 kinds of food coal-tar dyes (Tartrazine, Sunset Yellow FCF, Amaranth, New Coccine, Fast Green FCF, Brilliant Blue FCF and Indigo Carmine) was developed by the use of high performance liquid chromatography (HPLC) under the following operating conditions: Zipax SAX (strong anion exchange type resin) column; gradient elution system of 0.01M sodium borate solution and 0.1M sodium perchlorate solution; UV detection at 254nm as well as at the maximal absorbance wavelength of each dye. In order to try to introduce HPLC as a purity test in the Japanese Standards of Food Additives (JSFA), a regulation system for organic matter impurities is proposed based on total contents of starting material residues and uncombined intermediates expressed as the amount of sulphanilic acid, while total contents of subsidiary dyes were expressed as the amount of original dyes. By the use of the proposed method, a survey was carried out on 105 samples of coal-tar dyes that had passed the government product examination during 1978-1980.
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