Abstract
After renal transplant, surgical, infection complications, as well as graft rejection may occur; early detection through non-invasive markers is the key to change therapy and avoid biopsy. The aime of the study is to determine urine protein profiles in patients undergoing renal transplant with complications and detect its variation when therapy is modified. Urine samples were collected from patients prior the transplant and various postoperative stages. Urinary protein profiles were obtained by peptide labeling using isobaric isotopes for relative quantification (iTRAQ(®)). A total of 22patients were included, of whom 12developed post-transplant complication: 2 with graft rejection (one male and one female) and 10 (6males and 4females) in the group of post-transplant infections. Using iTRAQ(®) 15/345 and 28/113proteins were identified and fulfilled the acceptance criteria, in graft rejection and post-transplant infections group, respectively. Albumin was the only protein found in both groups, the remaining proteins were different. The 5proteins with higher scores in graft rejection were: alpha-1-microglobulin, 5'-nucleotidase cytosolicIII, retinol-binding protein4, membrane protein palmitoylated 4,and serine carboxypeptidase, while post-transplant infections were: mitochondrial acetyl-coenzymeA synthetase, putative adenosyl homocysteinase2, zinc finger proteinGLIS1, putative protein FAM157B, and zinc finger protein615. It remains to elucidate the involvement of each of these in patients with renal transplantation.
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