Abstract
52 known stock strains of yeast-like fungi were tested for thiaminase activity and the following results were obtained.1. Of the 51 strains tested, 16 (30per cent) decomposed more than 50per cent, 9 (18per cent) 30-50per cent, while 27 (52per cent) less than 30per cent of the thiamine added at pH 6.5 and 45° in 2 hours in the presence of 3γ thiamine.2. The thiaminase activity of representative strains, Trichosporon cutaneum 0174, Torula lactis 0398, Torulopsis xylinus 0454, Cryptococcus albidus 0378 and C4 strain of Yonezawa and Aoki was studied and found that the optimum pH was 6.0 and the optimum temperature 40°. Torulopsis xylinus, however, differed in that the optimum pH was 6.5 and the optimum temperature 60°.3. Determination of the thiaminase potency according to Fujita showed that C4 strain had the highest value, 146. Cryptococcus albidus 0378 had the value, 44.5 and Trichosporon cutaneum 0174, 30.5, only one-fourth that of C4.4. It is concluded that thiaminase is widely distributed in yeast-like fungi.
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