Abstract
Interpreting signals from the endogenous phytohormone trans-zeatin, which promotes cell division and differentiation, is essential for understanding plant growth and development. In this work, a supramolecular biosensor consisting of β-cyclodextrin (β-CD) and aggregation-induced emission-enhancing organic molecules (AIETPA) is reported. The trans-zeatin aptamer (Apt) competes with the AIETPA for preferential access to the β-CD due to the affinity difference between the host and the guest. Conversely, the presence of trans-zeatin enables the Apt to dissociate from the β-CD, as it forms a more favorable affinity with the trans-zeatin. This release of Apt from the β-CD allows AIETPA to occupy the β-CD cavity as a guest, resulting in fluorescence due to the restricted intramolecular motion (RIM). The sensor developed in this work exhibits high sensitivity (3 nM) and selectivity for trans-zeatin. More importantly, this work can monitor the total bioactivity signal of plant endogenous trans-zeatin through in situ fluorescence imaging, which holds great significance for chemical biology research.
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