Design, structural characterization, DNA interaction studies, antibacterial, antioxidant, and cytotoxicity studies of Co(II), Ni(II), Cu((II), Zn((II) complexes containing 2-methoxy 5-trifluoromethyl benzenamine Schiff base

Abstract

The bivalent Co(II), Ni(II), Cu(II), and Zn(II) complexes (A, B, C, D) of novel Schiff base ligand such as 2-((E)-(5-(trifluoromethyl)-2-methoxyphenylimino)methyl)-4-chlorophenol (HL1) have been synthesized. The structural analysis have been carried by elemental analysis, NMR, FTIR, ESI-mass, UV–Visible, ESR, TGA and these results confirmed that Ni(II) and Cu(II) complexes were exhibit square planar geometry, whereas Co(II) and Zn(II) complexes exhibits octahedral geometry. DNA binding interaction studies such as UV absorption, viscosity, fluorescence techniques revealed that the mode of binding of complexes with DNA is intercalative mode. DNA cleavage studies revealed that all the complexes were cleaved into Form I & II. The pathological studies of all the complexes were screened against microbial strains in-vitro, which revealed that Cu(II) complexes are more potent compared to other complexes and free ligands. The antidiabetic and antioxidant activity studies revealed that Cu(II) complex exhibit slight higher activity. Cytotoxicity studies on cell lines (KB3 & MCF7 cell lines) revealed that the all synthesized complexes were showed superior activity compared to the ligand.