Abstract
There is a growing need for anti-microbial materials in several biomedical application areas, such are hernia, skin grafts as well as gynecological products, owing to the complications caused by infection due to surgical biomaterials. The anti-microbial effects of silver in the form of nanoparticles, although effective, can be toxic to surrounding cells. In this study, we report, for the first time, a novel biomedical application of Ag0.3Na1.7La2Ti3O10-layered perovskite particles, blended with poly(L-lactide-co-glycolide) (PLGA), aimed at designing anti-microbial and tissue engineering scaffolds. The perovskite was incorporated in three concentrations of 1, 5, 10 and 15 w/w% and electrospun using dimethylformamide (DMF) and chloroform. The morphology of the resultant nanofibers revealed fiber diameters in the range of 408 to 610 nm by scanning electron microscopy. Mechanical properties of perovskite-based nanofibers also matched similar mechanical properties to human skin. We observed impressive anti-microbial activity, against Gram-negative, Gram-positive bacteria and even fungi, to Ag0.3Na1.7La2Ti3O10 in powder as well as nanofiber-incorporated forms. Furthermore, cytotoxicity assay and immunocytochemistry revealed that perovskite-based nanofibers promoted the proliferation of human dermal fibroblasts whist maintaining normal cellular protein expression. Our study shows that perovskite-nanofibers have potential as scaffolds for biomedical applications with anti-microbial needs.
Highlights
Anti-microbial materials are gaining more and more interest in the scientific community [1]
Synthesis and analysis of two layered perovskites and their anti-microbial properties were evaluated in order to ensure that anti-microbial properties of this novel material are induced by the presence of silver ions in the lattice
The antibacterial activity was measured against E. coli, P. aeruginosa, K. pneumoniae, S. saprophyticus and C. albicans, and scaffolds PLGA-10P and PLGa-15P were found to inhibit bacteria growth, dependent on perovskite concentration
Summary
Anti-microbial materials are gaining more and more interest in the scientific community [1]. Development of antibiotic resistant strains as well as new insight into wound healing mechanisms on a cellular level have allowed for the development of new materials that protect wounds from infection, but actively fight pathogens around the wound and stimulate tissue for self-regeneration. Those materials include various polymeric structures with proteins, metal oxides, metal nanoparticles particles as well as antibiotics and growth factors. A number of materials with perovskite structures have been proposed as novel materials due to its unique electric and magnetic properties. The application of perovskite in tissue engineering has not been explored
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