Abstract
In this work, we report novel amperometric urea biosensor based on immobilization of Urease enzyme onto self-assembled polyamidoamine grafted multiwalled carbon nanotube (MWCNT-PAMAM) dendrimers (from G1, first generation up to G5) on Au electrode. Bio-sensing electrode’s performance parameters such as optimum pH, temperature, and applied potential together with reproducibility were investigated. The electrode based on fifth generation of MWCNT-PAMAM and urease enzyme showed excellent performance in urea detection at an applied potential of 0.45V with short response time of 3s, wide linear range of 1–20mM, detection limit of 0.4mM, and sensitivity of 6.6nA/mM. No significant response was detected in the presence of possible interferon molecules. Non-diluted human blood plasma has been used for real sample application where electrode showed excellent performance in urea detection.
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