Design Formulation Ointment and Wound Healing Effectiveness of Onchidiid Slug (Onchidium typhae) Extract Ethanolic Assisted by Design Expert Software

Abstract

Research on onchidiid slugs has been conducted regarding their antibacterial activity and wound healing abilities. Objective: This study aims to design the ointment formula as an effective drug delivery base for wound healing. The formula design was obtained using the mixture design method using Design Expert software version 13.0. The proposed and optimum formulas were then evaluated for their physical properties (organoleptic, homogeneity, spreadability, adhesion, and pH). The incision wound was made with a wound length of 1.5 cm with a depth of 0.2 cm on the mouse’s back. The assay group was divided into several groups. Group I is the positive control treated with betadine ointment. Group II is the negative control treated with an ointment base without active extract. Group III is the group given crude extract. Groups IV, V, and VI are the treatment groups’ optimum formula of onchidiid slug ethanol extract ointment: 1, 3, and 5%, respectively. The intervention for each group was carried out for ten days with smearing once a day. The design results in optimal formulas with the composition of adeps lanae and vaseline album (9,566:70, 344) with a desirability value of 0.934. Tests for adhesion power, spreadability, and optimum formula pH meet the requirements for a good ointment. An ointment containing 1% onchidiid slug extract showed the best wound-healing effectiveness. This formula is 88.31% effective in wound healing. Onchidiid slug ethanol extract can be designed into an effective ointment for wound healing assisted by the mixture design method using Design Expert software.