Abstract
Two chemometric methods were developed for the simultaneous determination of telmisartan and hydrochlorothiazide. The chemometric methods applied were principal component regression (PCR) and partial least square (PLS-1). These approaches were successfully applied to quantify the two drugs in the mixture using the information included in the UV absorption spectra of appropriate solutions in the range of 200-350 nm with the intervals Δλ = 1 nm. The calibration of PCR and PLS-1 models was evaluated by internal validation (prediction of compounds in its own designed training set of calibration) and by external validation over laboratory prepared mixtures and pharmaceutical preparations. The PCR and PLS-1 methods require neither any separation step, nor any prior graphical treatment of the overlapping spectra of the two drugs in a mixture. The results of PCR and PLS-1 methods were compared with each other and a good agreement was found.
Highlights
Telmisartan (TEL) is angiotensin-II receptor antagonist used in the treatment of hypertension.[1]
The aim of this paper is to investigate the ability of PLS-1 and principal component regression (PCR) methods to quantify a binary mixture of TEL and HCZ with overlapping UV spectra and to apply the optimized models[19] in pharmaceutical preparations
The simultaneous determination of TEL and HCZ in Chemometric assisted spectrophotometric methods the tablet by conventional, derivative, and derivative ratio spectrophotometric methods is hindered by strong spectral overlap throughout the wavelength range
Summary
Telmisartan (TEL) is angiotensin-II receptor antagonist used in the treatment of hypertension.[1] Hydrochlorothiazide (HCZ) is one of the oldest and widely used thiazide diuretic.[2] Many analytical methods were developed for its determination either alone[3,4] or in combination with other antihypertensive drugs. These methods include spectrophotometry,[5,6,7] second derivative and first derivative of ratio spectra[8] absorbance ratio and first derivative,[9] polarography,[10] flow injection analysis,[11] HPLC,[12,13,14] and HPTLC.[15] The literature revealed some methods which include spectrophotometry[16] for the simultaneous determination of both the drugs. Control analysis on pharmaceutical preparations using the multivariate calibration method have been proved to be a valid alternative to HPLC.[18]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
