Design and fabrication of a three layered microfluidic device for lab on a chip applications

Abstract

This paper describes the design and fabrication of a microfluidic device for lab on a chip (LOC) applications. Homogenous fluid coverage in the detection well was studied using two and three layer LOC prototypes. Top and middle layer of the device were made of polydimethylsiloxane (PDMS) and bottom layer (LB) with polyethylene terephthalate (PET). The introduction of middle layer increased the depth of detection wells. Inorder to increase the hydrophilicity and hence the uniform well filling, the top layer was treated with PEG. The bonding between layers was performed by corona oxidation and uncured PDMS as adhesive. Carbon electrodes were printed on PET sheet and bonded with middle layer. The performance of LOC device was validated by analyzing the electrochemical response of redox species o-phenylenediamine.