Design and evaluation of hyaluronic acid coated plga nanoparticles of raloxifene hydrochloride for treatment of breast cancer

Abstract

Context In the present study, Hyaluronic acid coated Raloxifene loaded PLGA nanoparticles have been developed to improve the anticancer potential and reduce side effects associated with the drug. Aim and Objectives The investigation was aimed to formulate and optimize Raloxifene hydrochloride loaded PLGA nanoparticles with surface modification using hyaluronic acid as a targeting moiety. To perform physicochemical characterization, in vitro cytotoxicity study (using MCF-7), in vitro drug release study and in vivo pharmacodynamic study of optimized formulation. Methodology Raloxifene hydrochloride (RALH) loaded PLGA nanoparticles were prepared by nanoprecipitation technique, followed by surface modification with hyaluronic acid. Formulation was optimized by using 23 factorial design and characterized by physicochemical, in vitro drug release, in vitro cytotoxicity studies and in vivo pharmacokinetics. Results and discussion The particle size, PDI, zeta potential, entrapment efficiency and loading capacity of spherically shaped RALH loaded nanoparticles was 207.3 ± 4.2 d.nm, 0.218 ± 0.127, -28.3 mV, 43.75 ± 1.2% and 7.55 ± 1.14% respectively. The in-vitro drug release showed sustained release and followed Korsemeyer-Peppas model with non fickian release pattern. The in-vitro cytotoxicity study of drug loaded NPs by MTT assay on MCF-7 breast carcinoma cell showed anti-cancer activity after 48 hrs of treatment. Conclusion The results of the present investigation suggested that RALH loaded HA modified PLGA nanoparticles showed sustained drug release with anticancer activity and can be promising approach for treatment of breast cancer.