Abstract
A flow-through mini-reactor containing immobilized glucose oxidase (GOD) was designed, developed and evaluated. The immobilization of glucose oxidase directly on an electrode surface gives rise to very high losses in its activity whereas immobilization on a nylon support was found to be much more satisfactory. The mini-reactor, containing the covalently immobilized GOD, was coupled to the inlet of a wall-jet cell electrochemical detector, where the hydrogen peroxide produced by the enzymatic reaction was detected. This glucose sensor, mini-reactor plus wall-jet cell electrochemical detector, showed a detection limit of 5 μmol dm −3, good linearity and a very short response time and enabled us to study the kinetics of the enzyme immobilized on a porous support. The covalently immobilized enzyme has a lifetime of around one month. Application to the determination of glucose in blood plasma is described.
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