Abstract
In this study, we reported on a novel mimetic peroxidase, deuterohemin–Ala–His–Glu, (Dh–A–H–E). The kinetic parameters of Dh–A–H–E suggested that it was a mimetic peroxidase and followed the ping–pong mechanism. Compared to horseradish peroxidase, Dh–A–H–E exhibited excellent stability when tested at different pH and temperatures, and using different organic solvents. Based on our above results, a new method using Dh–A–H–E has successfully been developed for the fast and quantitative detection of trace amounts of glucose.
Highlights
Horseradish peroxidase (HRP) is a naturally occurring enzyme that has been widely applied in organic synthesis and biotransformation [1]
The stability of Dh–A–H–E is crucial for extending its potential applications
These results demonstrated that Dh–A–H–E had excellent stability and was very suitable (Figure 5d)
Summary
Ye Yuan 1 , Jia Xu 1 , Zhenyu Zhao 1 , Hui Li 1 , Kai Wang 1 , Zhi Wang 1,2,3, * and Liping Wang 1,2,3, *. Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, Jilin University, Changchun 130012, China. Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun 130012, China. Received: 18 January 2019; Accepted: 7 February 2019; Published: 11 February 2019
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