Desfluranın Aynı Konsantrasyonda Fakat Farklı Akımlarda Kullanılması Oksidatif Stresi Değiştiştirir mi? Hayvan Deneyi

Abstract

It is known that inhalation anesthetics are effective in the formation of oxidative stress and free radicals. The purpose of this study is to investigate these effects of desflurane and to detect changes in different flows. The rats were divided into three groups (10 mice/group). The first group was the control group. In the second group, mice were sedated with ketamine and given desflurane at a concentration of 6% in a 100% oxygen flow at a flow rate of 8 lt / min in a glass box for 20 minutes. The third group was comprised of ketamine-sedated mice that were given desflurane at a concentration of 6% in a 100% oxygen flow at a flow rate of 2 lt/min in a glass box for 20 minutes. Biochemical parameters were measured using commercial kits. Serum, brain, liver tissue MDA and SOD levels were significantly higher in the high flow group than in the control and low flow group. Serum GPx levels were significantly lower in the high flow group compared to both groups. ADMA levels in the serum, liver, and brain tissue were significantly higher in the high flow group. There was no significant difference in serum 8-OHdG levels between all three groups. Brain and liver tissue 8-OHDG levels were higher in both groups than control group. It has been observed that the use of low flow fresh gas even in the same concentrations of desflurane anesthesia models decreases oxidative stress whereas the use of high flow increases oxidative stress.