Abstract
Esterases have been associated with insecticide resistance in several aphid species. This report documents existence of 3 est erase polymorphisms associated with insecticide resistance in the green bug, Schizaphis graminum (Rondani). Est erases were resolved using polyacrylamide gel electrophoresis and Rf values were calculated for the 6 est erase bands that resolved. The 3 est erase polymorphisms observed have been named no noticeable elevated est erase, pattern 1 and pattern 2. Est erase bands visible on the polyacrylamide gel appear to have no elevated activity in the no noticeable elevated est erase strain. A single est erase band appears to be elevated in pattern 1 individuals and 3 est erase bands appear to be elevated in pattern 2. After testing >;10,000 green bugs, no individual green bug has been detected with both pattern 1 and pattern 2 banding. It is unknown whether the 2 elevated est erase forms are allelozymes or isozymes. Surface-residue bioassay data are presented that support the hypothesis that these elevated est erases may be important in insecticide resistance.
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