Abstract
One new and one known species of the genus Aphelenchoides from Iran are studied. Aphelenchoides giblindavisi n. sp. is mainly characterized by having five lines in the lateral fields at mid-body, and a single mucro with several tiny nodular protuberances, giving a warty appearance to it, as revealed by detailed scanning electron microscopic (SEM) studies. The new species is further characterized by having a body length of 546 to 795 μm in females and 523 to 679 μm in males, rounded lip region separated from the rest body by a shallow depression, 10 to 11 μm long stylet with small basal swellings, its conus shorter than the shaft ( m = 36-43), 52 to 69 µm long postvulval uterine sac (PUS), males with 16 to 18 μm long arcuate spicules, and three pairs of caudal papillae. The new species was morphologically compared with two species of the genus having five lines in the lateral fields namely A. paramonovi and A. shamimi and species having a warty-surfaced mucro at tail end and similar morphometric data ranges. The morphological features and morphometrics of the second studied species, A. helicus , agreed well with the data given for the type population. However, detailed study of fresh females revealed it has three drop-shaped stylet knobs and long PUS, making it typologically similar to the genus Robustodorus , meriting its taxonomic revision, i.e., transferring to it. In molecular phylogenetic analyses using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences, the new species formed a clade with A. heidelbergi in both SSU and LSU D2-D3 trees. The species A. helicus , however, clustered inside a well-supported clade of the genus Robustodorus in both trees, corroborating its newly proposed taxonomic placement as Robustodorus helicus n. comb.
Highlights
The genus Aphelenchoides Fischer, 1894, has around 175 nominal species (Mobasseri et al, 2018)
During our nematological surveys conducted in northern provinces of the country, two populations of the genus were recovered from Asalem forests in Gilan province, and a forest in Semnan province in north of Iran
Morphometric and phylogenetic studies, especially detailed scanning electron microscopic (SEM) studies, revealed the first species belongs to an unknown species, described as Aphelenchoides giblindavisi n. sp
Summary
The genus Aphelenchoides Fischer, 1894 (family Aphelenchoididae Skarbilovich, 1947), has around 175 nominal species (Mobasseri et al, 2018). The species Tylaphelenchus christinae Lieutier and Laumond, 1978 was transferred to the genus Aphelenchoides (Pedram et al, 2018a, 2018b) and two species A. subtenuis (Cobb, 1926) Steiner and Buhrer, 1932 and A. arachidis Bos, 1977 have been transferred to the genus Robustodorus Andrássy, 2007 (Kanzaki et al, 2018). The latter action, in revising the taxonomic placement of those two species, was supported by both traditional and molecular criteria. Present study aims to (i) describe Aphelenchoides giblindavisi n. sp. and characterize it using morphological and molecular data and (ii) to revise the current taxonomic status of A. helicus using newly observed morphological traits and molecular phylogenetic criteria
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