Description of Antibiotic Susceptibility Pattern Analysis on Methicillin-SusceptibleStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusClothing Gene Panton-Valentine Leukocidin from Clinic Isolate

Abstract

Staphylococcus aureusis the most common cause of health care acquired infection and has a high rate of morbidity and mortality.S. aureushas a number of virulent factors that facilitate these bacteria to adhesion to the tissues of the host, avoiding the body’s defense system, and damaging the host cell. PVL cytotoxin is one of the virulent factors that causes hypervirulent inS. aureus.The infections caused are varied, ranging from severe skin infections, peneumonia, and sepsis. To obtain a description of the antibiotic susceptibility pattern in Methicillin-sensitiveStaphylococcus aureus(MSSA) and Methicillin-resistantStaphylococcus aureus(MRSA) carriers of the pvl gene that causes infection. IsolateS. aureusderived from clinical isolates of inpatients who have identified and tested phenotypic antibiotic susceptibility using BD Phoenix TM Automated Microbiology System or Vitek2 Compact bioMérieux system followed by genotypic testing using PCR to detect the presence of the pvl gene. Of 85S. aureusisolates, 65 isolates (76.5%) included MSSA bacterial strains and 20 isolates (23.5%) including strains of MRSA bacteria. Of the 65 isolates of MSSA bacterial strains, 9 isolates (13.84%) had the pvl gene, and of the 20 isolates of the MRSA strain, 1 isolate (5%) had the pvl gene. The susceptibility of non betalactam antibiotics to MSSA virus strains of pvl gene carriers ranged from 77.8%-100%. MSSA bacterial strains that did not carry the pvl gene also showed a non-betallactam antibiotic susceptibility ranging from 69.1%-100%. The susceptibility of tetracycline antibiotics to both MSSA strains of both pvl and non-carriers of pvl genes was 55.6% and 39.3% respectively. There was no significant difference between the antibiotic susceptibility pattern in the MSSA carrier strain of the pvl gene carrier and the proportion of the MRSA carrier strain of the pvl gene carrier.