Descending projections from the inferior colliculus to the dorsal cochlear nucleus in the cat: An autoradiographic study

Abstract

Descending auditory projections from different subdivisions of the inferior colliculus to the dorsal cochlear nucleus were investigated in experiments using the autoradiographic technique. Tritiated leucine injections confined to the pericentral nucleus of the inferior colliculus resulted in the appearance of dense grain clusters distributed over the outer fusiform cell and molecular layers of the ipsilateral dorsal cochlear nucleus. The pattern and distribution of dense grain clusters strongly resembled the central terminals of glomeruli described previously in the dorsal cochlear nucleus. Injections into the dorsal region of the central nucleus of the inferior colliculus led to a more diffuse distribution of grains over the middle and outer fusiform cell layer and over the innermost molecular layer of the dorsal cochlear nucleus on both sides. Dense grain clusters were also evident after these injections but they appeared to result from the concomitant injection into the overlying pericentral nucleus. Finally, injections of tritiated leucine into the ventral region of the central nucleus of the inferior colliculus (which included some cells of the dorsal nucleus of the lateral lemniscus) resulted in the heaviest labelling of the dorsal cochlear nucleus. Grains were distributed perisomatically and peridendritically around fusiform cells of the fusiform cell layer and giant cells of the deep dorsal cochlear nucleus on both sides. The results indicate that the pericentral nucleus and the more dorsal region of the central nucleus of the inferior colliculus establish overlapping connections with the outermost fusiform cell and molecular layers of the dorsal cochlear nucleus. Both sets of connections seem to be made principally with interneurons through glomerular and other inputs to scattered small cells which exist in these laminae. Since cortical and thalamic descending fibers directly innervate only the most dorsal regions of the inferior colliculus, it may be that this region of the tectum selectively mediates activity from these higher auditory centers. Such centers may indirectly influence fusiform cell response properties through collicular inputs to small cells of the dorsal cochlear nucleus that contact fusiform cells. A more substantial and direct projection was shown to arise from the ventral region of the inferior colliculus to innervate both the fusiform and giant cells. As such, the descending connections from the ventral inferior colliculus may be more likely to influence directly the output of both the fusiform and giant cells and, therefore, the projection of auditory information from the dorsal cochlear nucleus to higher levels.