Abstract
Optimal conditions for aerobic formation and anaerobic biohydrogenation of linoleic acid were determined in sheep rumen fluid with added olive oil fatty acids. Both activities were highest at 38C. Formation of linoleic acid exhibited maxima at pH 5.0 and 7.5, and hydrogenating activity was highest at pH 6.0.Washed rumen bacteria suspended in phosphate buffer showed a requirement for reduced pyridine nucleotides in the aerobic formation of linoleic acid. Cysteine, quinol, and sucrose enhanced anaerobic hydrogenation and induced saturation of linoleic acid even under aerobic conditions. Addition of l-14carbon labeled oleic and linoleic acid revealed a bidirectional flux of label between the monoene and diene fraction upon aerobic incubation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
