Abstract
Ten methods of derivatization of phenolic acids and the suitability of the derivatives so obtained for non-polar (SE-30, OV-1) capillary gas chromatographic (GC) quantitation with the flame ionization detection (FID) and electron-capture detection (ECD) have been studied. The phenolic acids included three benzoic and three trans-cinnamic acids, both series consisting of 4-hydroxy, 4-hydroxy-3-methoxy and 4-hydroxy-3,5-dimethoxy derivatives. The trimethylsilyl (TMS) ethers of the phenolic acid methyl esters, the TMS ethers of the phenolic acid TMS esters and the heptafluorobutyrates of the phenolic acid methyl esters were found to be suitable for quantitation with FID and the first mentioned derivatives are preferred. For quantitation with ECD, the perfluorobutyrates of the phenolic acid methyl esters were the best derivatives. The quantitation of phenolic acids by GC—FID is suitable for samples with concentrations in the range of 0.01–10 μg per injection. The concentrations applicable to GC—ECD quantitation are much lower, 0.05–1 ng per injection.
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