Derivation of feline vaccine-associated fibrosarcoma cell line and its growth on chick embryo chorioallantoic membrane – a new in vivo model for veterinary oncological studies

K Zabielska,M Król,T Motyl,R Lechowski,I Dolka,A Żbikowski,K M Pawłowski

doi:10.1007/s11259-012-9535-9

K Zabielska, M Król + Show 5 more

Open Access

https://doi.org/10.1007/s11259-012-9535-9

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Abstract

Feline vaccine associated fibrosarcomas are the second most common skin tumor in cats. Methods of treatment are: surgery, chemotherapy and radiotherapy. Nevertheless, the usage of cytostatics in feline vaccine associated sarcoma therapy is limited due to their adverse side effects, high toxicity and low biodistribution after i.v. injection. Therefore, much research on new therapeutic drugs is being conducted. In human medicine, the chick embryo chorioallantoic membrane (CAM) model is used as a cheap and easy to perform assay to assess new drug effectiveness in cancer treatment. Various human cell lines have different tumors growth on CAM. In veterinary medicine such model has not been described yet. In the present article derivation of feline vaccine associated fibrosarcoma cell line and its growth on CAM is described. The cell line and the tumor grown were confirmed by histopathological and immunohistochemical examination. As far as we believe, this is the first attempt to create such model, which may be used for further in vivo studies in veterinary oncology.

Highlights

Feline vaccine-associated fibrosarcomas are the second most common kind of skin tumors found in cats
Cell lines derived from vaccine-associated feline sarcoma were strongly positive for vimentin (+++) (Fig. 2a), some cells were positive for smooth muscle actin (+,+/−) (Fig. 2b), single cells positive for desmin (+,+/−) (Fig. 2c) and for cytokeratin (+/−) (Fig. 2d)
The tumor had smooth surface, ovoid to spherical shape and was strongly attached to the chorioallantoic membrane (CAM). It was located not exactly in the silicon ring, but a few milimeters out of it, either the tumor cells had spread through the vessels or the silicon ring position had slightly moved during embryogenesis and growth of the CAM and chick embryo

Summary

Introduction

Feline vaccine-associated fibrosarcomas are the second most common kind of skin tumors found in cats. The exact mechanism of the formation of fibrosarcomas in cats is unknown, but it is suspected that the overexpressed inflammation of skin in the place of injection changes into neoplasm. According to the latest reports the genetic predisposition may be important (Nambiar et al 2001). It has been proven that cats injected many times in the same place (especially in the intrascapular region) are more likely to develop tumors. According to the newest guidelines published by the Vaccine Associated Feline Sarcoma Task Force from the U.S cats should be injected near to pelvic limbs changing sides, because in severe cases leg amputations can be performed. Diagnosis is made based on the history, clinical examination of the patient and results of

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