Abstract
Protein extracts of Phycomyces blakesleeanus are tested for peroxidase activity by guajacol-benzidine after fractionation by polyacrylamide-discelectrophoresis. Only a minor part of the protein zones shows very low peroxidase activity. The indole-3-acetic acid oxidase activity is determined spectrophotometrically. Fungus cultures from different growth conditions (light, dark, zinc sufficiency, and zinc deficiency) were used after separation in mycelium and sporangiophores. Sporangiophores have a noticeably higher indole-3-acetic acid oxidase activity than mycelium. Cultures with stronger sporangiophore growth evidently have lower indoleacetic acid oxidase activity than those with weaker sporangiophore development. We detected only minor indoleacetic acid oxidase activity in shake cultures with suppressed sporangiophore development. The inhibition of indoleacetic acid oxidase activity by gallic acid could be proved by in vitro tests.
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