Abstract
BackgroundThis study investigated the relationship between ethanol intake in rats and the resulting level of ethyl glucuronide (EtG) in rat hair. MethodsRats (n=50) consumed a 10% ethanol solution for 4 weeks, then EtG was extracted from samples of their hair using a novel extraction procedure involving freezing and thawing. The EtG concentration was measured using gas chromatography and mass spectrometry. The animals voluntarily drank ethanol, with daily consumption in most rats exceeding 5g/kg b.w. The silylated EtG was stable for at least 28h. The limit of detection was 0.03ng/mg, and the limit of quantification was 0.1ng/mg. ResultsHair samples from rats that consumed ethanol had EtG levels ranging from 0.17–20.72ng/mg in female rats and 0.15–13.72ng/mg in males. There was a correlation between the amount of alcohol consumed and the EtG levels in hair from female (p<0.01), but not male, rats. ConclusionThe method presented allows detection and quantification of EtG in rat hair. We also observed differences in EtG deposition in male and female rats.
Paper version not known (
Free)
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call