Deposition Behavior of Self-Assembled Monolayers and Bacteria on Metallic Surfaces Using an Electrochemical Quartz Crystal Nanobalance

Abstract

This paper presents an investigation on the deposition behavior of self-assembled monolayers (SAMs) and bacteria on titanium and gold surfaces using an electrochemical quartz crystal nanobalance (EQCN). The tests included alkanethiol and alkanesilane and three bacteria, namely: E. coli, P. fluorescens and K. aerogenes. The mass change with respect to immersion time was measured by EQCN. The results showed SAM formation on both titanium and gold surfaces, but SAM formation on gold was generally higher by 26-74% as compared to that of titanium. On the other hand, bacteria also adhered well on the two metal surfaces. E. coli and P. fluorescens had high affinity on gold and titanium surfaces, respectively, while K. aerogenes was more adherent to titanium. The results showed that titanium and gold are good metals for biomaterials yet at the same time, their bioinert property provide excellent condition for bacterial adhesion. Therefore, there is a need for proper surface preparation to optimize the use of titanium and gold as biomaterials.