Abstract
The role of intracellular free Ca 2+ in muscarinic-receptor linked depolarization of SH-SY5Y neuroblastoma cells has been determined by using the bisoxonol membrane potential probe DiBaC 4−(3) and intracellular Ca 2+ indicator fura-2-respectively. Carbachol and the Ca 2+ ionophore, ionomycin, at concentrations which caused similar rises in intracellular Ca 2+ increased the bisoxonol fluorescence (depolarization) to the same extent. The membrane potential responses, but not the changes in intracellular Ca 2+, were dependent on extracellular Na +. Ionomycin depletion of intracellular Ca 2+ with EGTA and ionomycin or loading the cells with a Ca 2+ buffer, BAPTA, reduced the carbachol-induced depolarization. The results suggest that a rise in intracellular Ca 2+ may cause depolarization through an increase in the Na + permeability.
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