DEPENDENCE OF METABOLIC DISORDERS ON ALDOSTERONE SYNTHASE CYP11B2 (-344C/T) GENE POLYMORPHISM IN HYPERTENSIVE PATIENTS

Abstract

Objective: The aim of the study was to analyze the association of CYP11B2 (-344C/T) gene polymorphism with lipids profile and aldosterone level changes in patients with essential arterial hypertension (EAH). Design and method: One-hundred subjects with EAH and target-organ damaging (2nd stage), moderate, high or very high cardiovascular risk were involved in the case-control study. Among them 79.0% females and 21.0% males, mean age 59.87 ± 8.02; disease duration from 6 to 25 years. Control group included 48 practically healthy persons of relevant age. Gene polymorphism of aldosterone synthase gene CYP11B2 (-344C/T) was examined by polymerase chain reaction. Lipids’ profile in serum was studied by total cholesterol (TC), high- and low density levels cholesterol (HDL-C, LDL-C), triglycerides (TG) concentration and atherogenic index (AI). Aldosterone plasma concentration was assessed by ELISA. Results: The TG level and atherogenic index were higher in T-allele carriers than in CC-genotype of CYP11B2 gene: in patients by 22.61–56.2% (p < 0.05), in control group by 31.16–77.42% (p < 0.05). Contrary, the HDL-C level was lower T-allele carriers in both groups: by 12.23% and 12.95% (p < 0.05) in patients and by 18.34% and 27.22% (p < 0.05) in control group, respectively. Significant differences in lipids profile data between patients and control groups depending on genotypes were not observed. Aldosterone concentration elevated also in T-allele carrier’s patients by 53.62% (p < 0.001) and 26.90% (p = 0.015), than in CC-genotype and was 2.14–2.72 times higher, than in control group (p < 0.001). TG level in CC-genotype males was higher, than in CC-genotype’s women by 68.57% (p = 0.028), but in TT-genotype males HDL-C was reliably lower by 18.40% (p = 0.008) than in TT-carriers’ women with higher atherogenic index by 25.84% (p = 0.031). Otherwise, aldosterone concentration was certainly higher in women, than in men regardless the genotypes of CYP11B2 gene. But aldosterone level prevailed in T-allele carriers of both genders over CC-genotype patients’ data: by 27.01% and 49.64% (p < 0.05) for women and by 20.93% and 43.11% (p < 0.05) for men. Conclusions: Thus, TT-genotype or T-allele of aldosterone synthase gene CYP11B2 (-344C/T) associated with higher blood level of triglycerides and aldosterone concentration (especially in females), elevated atherogenic index and lower HDL-C rate (preferably in males).