Abstract
We have studied deoxyribonucleic acid (DNA) repair synthesis in permeable, confluent normal and repair- deficient human fibroblasts using (3H)dCTP, BrdUTP, dATP, and dGTP as substrates. In this system repair synthesis occurred as judged by the following criteria: (1) labeled nucleotides were incorporated into parental DNA (p = 1.73 g/cm3 in alkaline CsCl); (2) incorporation into parental DNA was negligible in the absence of damage and increased 10- 20-fold in normal cells following exposure to ultraviolet ra- diation (UV) or the direct-acting chemical carcinogen N- acetoxy-2-(acetylamino)fluorene; and (3) damage-dependent DNA synthesis was absent in preparations made from excision repair-deficient human diploid fibroblasts (xeroderma pig- mentosum cells, complementation group A). The reaction was
Published Version
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