Abstract
Subtribe Coelogyninae (Epidendroideae, Orchidaceae) comprises of 16 genera. The present study is undertaken to develop DNA barcodes of subtribe Coelogyninae using rbcL. It reveals the sectional delineation and its relationship with Pholidota. DNA barcoding is a technique which helps identification of species even if only small fragment of organism is available at any stage. The rbcL (Rubisco large subunit) from chloroplast genome is tested for effective barcoding. The interspecific divergence values and species discrimination rates were calculated by Kimura 2 parameter using MEGA 4.0. The rbcL with average interspecific divergence values yielded 72.72 % species resolution, thus it could distinguish all the species of Coelogyne and Pholidota. Invariably the genus Pholidota shows close affinity with Coelogyne. This supports the inclusion of genus Pholidota in the subtribe Coelogyninae of tribe Coelogyneae.
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