Abstract
Deoxycytidine stimulated the incorporation of 3H-lysine in nucleoprotein extracted by 2 M NaCl. Further characterisation steps showed that an important part of the radioactivity was confined to an acid-extractable or trypsin-released acid-soluble fraction, indicating a dibasic amino acid rich protein. Increase of 3H-lysine incorporation into this fraction was stimulated by CdR at 0.5 mM while increase in specific radioactivity of total nucleoprotein extended up to 1.5 mM. While incomplete separation of follicle cells and oocyte content is always the limitation inherent in oogenesis study, results from sucrose gradient centrifugation indicated that at least part of the radioactivity was found in particles other than follicle cell and ovarian tissue nuclei. CdR may help to build up an increased deoxyriboside triphosphate pool through the enzymatic reduction of riboside diphosphates [16]; thus promoting cDNA neosynthesis, which in turn stimulates an increase in basic protein synthesis. A direct relationship between the synthesis of cDNA and cytoplasmic basic protein is under investigation.
Published Version
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