Abstract
Sea urchin ( Strongylocentrotus purpuratus) DNAs from morula and blastula stage embryos and from sperm were examined, by hybridization to [ 3H] polyuridylic acid, for the proportion of DNA sequences rich in deoxyadenylate. By this criterion, it was found that, relative to sperm DNA, morula DNA has 65% less deoxyadenylate-rich DNA. Blastula DNA has 1.7 times as much deoxyadenylate-rich sequence as does morula DNA. Fractionation of these DNAs on neutral and alkaline cesium chloride density gradients indicates that the differences in the deoxyadenylate-rich content of the DNAs pertains predominantly to main density band DNA. Fractionation on cesium salt gradients also obviates the possibility that the measured differences in the DNAs result from preferential loss of satellite DNA during isolation of bulk DNAs.
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