Abstract
The effect of population density on cellular uptake of adenine, leucine, thymidine, and uridine was studied in monolayer cultures of a mammalian cell line not subject to density-dependent inhibition of growth. Experiments were conducted during the logarithmic phase of the growth cycle using cultures of equal age but at different cell densities to avoid effects of aging or of the transition to stationary-phase growth. Results indicate that uptake of leucine is independent of cell density, whereas uptake of adenine, thymidine, and uridine vary inversely with density. At higher densities incorporation of radioactive adenine, thymidine, and uridine into cellular macromolecules is reduced to approximately the same extent as uptake suggesting that utilization is limited by entry into the cell.
Published Version
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