Abstract
Objective: To develop a validated high-performance thin layer chromatography (HPTLC) method for the quantitative estimation of Lapachol in a Soxhlet extracted bark of Tecomella undulata seem (T. undulata). Methods: The bark of T. undulata was extracted with chloroform by Soxhlet apparatus. The separation was achieved on a silica-gel 60 F254 HPTLC plate using toluene-ethyl acetate–glacial acetic acid (8.5:1.5:0.02 v/v/v) as a mobile phase. Densitometric analysis of Lapachol was carried out in absorbance mode at 254 nm.Results: The proposed method was accurate for the separation and resolution between peaks of the standard and Lapachol (5.04µg) with Rf value 0.77. Calibration curves were found to be linear over the concentration range (10-130µg) for Lapachol and correlation coefficient over (R2= 0.9973), indicating an excellent correlation between peak areas and concentrations of the marker compound. The experimentally derived LOD and LOQ for Lapachol were determined to be 0.028 µg and 0.086 µg respectively and the developed HPTLC-UV method showed lower %RSD and SEM, value indicating the method to be precise, accurate and robust.Conclusion: The study concludes that HPTLC-UV validation method can be very efficient and promising technique for the identification and quantitative analysis of Lapachol from T. undulata bark. The statistical analysis of data indicates that the developed method is reproducible and specific.
Highlights
Tecomella undulata Seem (Bigoniaceae) is a tree species, locally known as Rohida found in Thar Desert regions of India and Pakistan, which is used as a hepatoprotective agent in various ayurvedic formulations such as Rohitakarishta, Rohitakadyachurna, Rohitakaghrita and Rohitakalauha [1]
A validated high-performance thin layer chromatography (HPTLC) method was developed for the quantitative estimation of Lapachol in a Soxhlet extracted bark of (T. undulata)
The linear correlation between peak area and applied concentration was found in the concentration range of 10-130 μg/ml for Lapachol
Summary
Tecomella undulata Seem (Bigoniaceae) is a tree species, locally known as Rohida found in Thar Desert regions of India and Pakistan, which is used as a hepatoprotective agent in various ayurvedic formulations such as Rohitakarishta, Rohitakadyachurna, Rohitakaghrita and Rohitakalauha [1]. Naphthoquinones are natural aromatic compounds that can be found in several plant families, as well as isolated from fungi, algae and bacteria Used for their dyeing properties, recently a variety of biological activities of these compounds has been reported [3]. HPTLC is often used as an alternative to HPLC for the quantification of plant products because of its simplicity, accuracy, cost-effectiveness and rapidity [17]. In this investigation, a validated high-performance thin layer chromatography (HPTLC) method was developed for the quantitative estimation of Lapachol in a Soxhlet extracted bark of (T. undulata)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Pharmacy and Pharmaceutical Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
