Denitrifying Bacteria Active in Woodchip Bioreactors at Low-Temperature Conditions.

Jeonghwan Jang,Gary W Feyereisen,Emily L Anderson,Carl J Rosen,Satoshi Ishii,Michael J Sadowsky,Rodney T Venterea

doi:10.3389/fmicb.2019.00635

Abstract

Woodchip bioreactor technology removes nitrate from agricultural subsurface drainage by using denitrifying microorganisms. Although woodchip bioreactors have demonstrated success in many field locations, low water temperature can significantly limit bioreactor efficiency and performance. To improve bioreactor performance, it is important to identify the microbes responsible for nitrate removal at low temperature conditions. Therefore, in this study, we identified and characterized denitrifiers active at low-temperature conditions by using culture-independent and -dependent approaches. By comparative 16S rRNA (gene) analysis and culture isolation technique, Pseudomonas spp., Polaromonas spp., and Cellulomonas spp. were identified as being important bacteria responsible for denitrification in woodchip bioreactor microcosms at relatively low temperature conditions (15°C). Genome analysis of Cellulomonas sp. strain WB94 confirmed the presence of nitrite reductase gene nirK. Transcription levels of this nirK were significantly higher in the denitrifying microcosms than in the non-denitrifying microcosms. Strain WB94 was also capable of degrading cellulose and other complex polysaccharides. Taken together, our results suggest that Cellulomonas sp. denitrifiers could degrade woodchips to provide carbon source and electron donors to themselves and other denitrifiers in woodchip bioreactors at low-temperature conditions. By inoculating these denitrifiers (i.e., bioaugmentation), it might be possible to increase the nitrate removal rate of woodchip bioreactors at low-temperature conditions.

Highlights

Nitrogen (N) and phosphorus (P) are the most important nutrients in fertilizers for agriculture
Woodchip bioreactors have demonstrated success in nitrate removal in many field locations (Christianson et al, 2012a), low water temperature during the cold seasons significantly limits bioreactor performance (Christianson et al, 2012b; David et al, 2016), which is likely related to the low metabolic activity of denitrifying microorganisms at low temperatures
To identify denitrifiers active in the woodchip bioreactors at relatively low-temperature conditions, we established a series of reproducible woodchip bioreactor microcosms to evaluate the following four treatments: (i) WINA, woodchip microcosm incubated with nitrate and acetate; (ii) WIN, woodchip microcosm incubated with nitrate but without acetate; (iii) WIA, woodchip microcosm incubated with acetate but without nitrate; and (iv) WI, woodchip microcosm incubated without nitrate and acetate

Summary

Introduction

Nitrogen (N) and phosphorus (P) are the most important nutrients in fertilizers for agriculture. Woodchip bioreactors have demonstrated success in nitrate removal in many field locations (Christianson et al, 2012a), low water temperature during the cold seasons significantly limits bioreactor performance (Christianson et al, 2012b; David et al, 2016), which is likely related to the low metabolic activity of denitrifying microorganisms at low temperatures. In addition to cold temperatures (

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