Abstract
Abstract Killing a clover sward for subsequent use of the area for grain production results in conditions favourable for denitrification. The large quantity of readily available carbon (C) stimulates microbial respiration which in tum causes the anaerobic conditions needed for denitrification. A wbite clover (Trifolium repens L.) sward containing 10 500 kg/ha of above- and below-ground biomass and 250 kg/ha of nitrogen (N) was killed by mouldboard plougbing, rotary boeing, shallow tillage, or with a berbicide. Soil samples were taken periodically to determine the accumulation of NH4Nand NO3-N. Cbambers were formed by driving 25 × 40 em metal frames 10 cm into the soil and covering with rigid sheets of clear plastic. Acetylene (C2H22) was injected into these chambers to achieve a 5–10% concentration in the headspace above the soil and block the reduction of nitrous oxide (N2O) to N2. Gas samples were taken periodically for N2O determination on a gas chromatograph. Soil nitrate accumulated in the general or...
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