Dendrobium sp. subcultures with instant Vacin Went media against many concentrations of fruit waste liquid organic fertilizer plus

Abstract

Orchids are ornamental plants in great demand by collectors and the public due to the varieties colors of Orchid flowers and the price. This study aims to determine the effect and obtain the concentration of the application of fruit waste liquid organic fertilizer plus on the growth and development of a Dendrobium sp. sub-culture on VW media in vitro. The research had been carried out from May to August 2022 at the Biotechnology Laboratory of Kupang State Agricultural Polytechnic. The study design used a single-factor complete randomized design consisting of six treatments and four replications. The tested factors were six level of concentrations of fruit waste liquid organic fertilizer plus instant VW media in vitro, which are: instant VW media; instant VW media + 2.5 ml/L; instant VW media + 5 ml/L; instant VW media + 7.5 ml/L; instant VW media + 10 ml/L; and instant VW media + 12.5 ml/L. The results showed that the best concentration of Fruit Waste Plus LOF for the growth of Dendrobium orchid explants is the treatment instant VW media + 7.5 ml/L LOF, which is shown by the time of bud emergence (10.75 days after planting), number of shoots (2.38), shoot height (3.5 mm), number of roots (1.38), and root length (2.5 mm).