Deletion analysis of genes regulating cold- and PEG-induced carbohydrate accumulation in hydroponically raised wheat seedlings

Abstract

Listen

Translate article

The mobilization of carbohydrates, especially sucrose, is considered very important during both the cold acclimation process and water stress, while sugars also promote floral transition and cold hardiness. Chinese Spring (CS) 5AL and 5DL deletion lines were studied for the physical assignment of the gene(s) regulating stress-induced sugar accumulation. To separate the effect of cold from that of water deprivation, the seedlings were raised in hydroponics, and apart from the cold, the effect of PEG-induced water stress was also evaluated in a time course experiment. The genes affecting stress-induced carbohydrate accumulation were assigned to the same chromosomal bins, which contain the vernalization genesVrn-A1andVrn-D1, on the long arms of chromosomes 5A and 5D, respectively. Sugar accumulation was found to be controlled byVrngenes in an epistatic manner at least at the beginning of the cold treatment. In the case of cold treatment,Vrn-A1proved to be more effective thanVrn-D1, while in the case of osmotic stress the gene assigned to the long arm of chromosome 5D seemed to be more effective at regulating sugar accumulation than its counterpart on 5A.