Değişik karbon ve azot kaynaklarının Macrolepiota procera ve Polyporus squamosus mantarlarının sıvı kültürde misel biyomas üretimi üzerine etkileri

Abstract

This study was carried out to investigate the effects of various carbon and nitrogen sources on mycelial biomass production of Macrolepiota procera and Polyporus squamosus, important edible mushrooms in Turkey, in submerged cultures. Seven carbon (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen sources ((NH4)2HPO4, NH4NO3, Ca(NO3)2, malt extract, peptone and yeast extract) were used in the study. The Sabouroud (SB) and Dextrose Yeast Extract (DYE) liquid media without nitrogen and carbon were considered as the control. All carbon and nitrogen sources promoted significantly (p<0.01) mycelial biomass production in M. procera and P. squamosus. The optimum carbon and nitrogen sources for mycelial biomass production showed changes depending on mushroom species. As a result, the most suitable carbon source for mycelial biomass production in M. procera was dextrose and followed by glucose, mannitol, sucrose and lactose, while the medium with xylose was in P. squamosus. Peptone and malt extract as a nitrogen source in M. procera gave the best result for the biomass production, whereas yeast extract was the most favorable nitrogen source in P. squamosus. The lowest mycelial biomass production for the both mushroom species was determined in the control medium without carbon and nitrogen. In conclusion, the basic informations obtained from this study could be useful in the optimization of submerged culture conditions and nutritional requirements for mycelial biomass production in M. procera and P. squamosus.