Abstract
Activity of various dehydrogenases was studied histochemically in fully developed, functional Sprague-Dawley rat molar pulps using a method of demineralization that conserves substantial enzyme activity. Odontoblasts, cells of the subodontoblastic cell-rich zone, other connective tissue cells of the pulp and cells of blood vessels and nerves showed varying degrees of activity for succinic, malic, isocitric, glutamic, lactic, α-glycerophosphate, β-hydroxybutyric, glucose-6-phosphate, NADH 2and NADPH 2- dehydrogenases. Especially marked reactions were noted in odontoblasts and cells of the sub-odontoblastic zone. From these results it can be inferred that the cells of the pulp possess the capacity for glycolysis and fatty acid metabolism, and also a functional citric acid cycle and pentose shunt.
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