Degradation of sulfadiazine and electricity generation from wastewater using Bacillus subtilis EL06 integrated with an open circuit system

Abstract

Antibiotics are one of the emerging pollutants that have drawn increased attention in recent years. In this study simultaneous production of electricity and bioremediation of sulfadiazine from the wastewater using microbial fuel cells (MFCs) and open circuit system were analyzed. LC-MS analysis revealed decreased sulfadiazine concentration in open circuit system and closed MFC chamber. In MFCs, 12.6 ± 1.3% removal efficiency of sulfadiazine was reached after 30 h and it improved 89.2 ± 2.1% after 100 h. The initial sulfadiazine concentration influenced on antibiotic removal in MFCs and antibiotic removal was increased up to 350 μg/L. At higher antibiotic concentration, reduced microbial activity results depleted antibiotic degradation. MFCs degraded sulfadiazine (350 μg/L) completely within 80 h of treatment. The maximum COD removal (91.9 ± 2.3%) was obtained in MFCs chamber and it was about 60.8 ± 2.7% in the open circuit chamber. The initial total phosphorus content of the wastewater fed with reactor was 1350 mg/L and it reduced considerably after treatment in MFCs system (89.9 ± 2.8%). Total microbial population was 4.5 × 105 CFU/mL after one day of treatment and it declined after five days due to the depletion of nutrients in the wastewater. In MFC electricity generation reached maximum within 60 h of treatment (1.28 ± 0.1 V). The electrogenic strain Bacillus subtilis EL06 was characterized from the MFC and sulfadiazine tolerance was analyzed. These findings demonstrated that MFCs are useful for the simultaneous bioremediation of wastewater and electricity generation.