Abstract
High-density cultures require operating below the critical threshold of shear stress, in order to avoid reducing the specific growth rate of the cells. When determining this threshold, direct inspection of the cells in flow provides insight into the conditions of shearing. Aim of this study was using a novel rheo-optical setup for the observation of cells in laminar shear flow and the determination of the critical shear stress required to damage them in their natural environment. Dunaliella salina cells were sheared and observed in flow for shear stresses of up to 90Pa, at ambient temperature, without adding thickeners. The critical shear stress was determined by fitting a hydrodynamics-based criterion to the experimental data on the percentage of deformed cells after shearing. Single cells, clusters and strings of cells were visible in shear flow. The strings formed at maximum shear stresses of 10Pa or higher. Cells lost motility for maximum shear stresses higher than 15Pa, and more than 80% of the cells were deformed at maximum shear stresses higher than 60Pa. The estimated critical shear stress was 18Pa. Shear stresses higher than 18Pa should be avoided when cultivating D. salina.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call