Abstract
Plants recognize certain microbial compounds as elicitors of their active defense mechanisms. In the present study, NUBS-4190, a synthetic bis-aryl-methanone compound elicited NO and ROS generation in potato suspension cultured cells and intact potato leaves. Hypersensitive cell death was found in these cultured cells and in potato leaves without the accumulation of phytoalexins in the tubers. Defense-related genes such as StrbohB, StrbohC, StNR1, StNR5, Sthsr203J and StPR1 were expressed in potato suspension cultured cells treated with NUBS-4190. Resistance against Phytophthora infestans also increased in NUBS-4190-treated potato leaves.
Highlights
Recognition of a pathogen by a plant is essential for the plant to activate the available biochemical and structural defenses against the pathogen
nitric oxide (NO) generation in a time-course experiment was assayed in suspension cultured cells of potato treated with 10 lg/mL NUBS-4190-treated using DAF-2DA-mediated fluorescence (Fig. 2a)
Increased NO generation was not found in 1 % (v/v) DMSO-treated potato leaves, rather basal fluorescence was detected by DMSO and did not differ statistically from the cPTIO treatment
Summary
Recognition of a pathogen by a plant is essential for the plant to activate the available biochemical and structural defenses against the pathogen. NO and target genes of NO contribute to the oligogalacturonide-triggered responses against Botrytis cinerea (Rasul et al 2012), NO participates as a partner of ROS in disease resistance to B. cinerea in Nicotiana benthamiana (Asai and Yoshioka 2009). This involvement of NO in plant disease resistance is related to its interplay with ROS, and cell death during hypersensitive response (HR) may result from the simultaneous and balanced production of NO and ROS (Zaninotto et al 2006). In the context of plant–pathogen interactions, NO is involved in the modulation of salicylic acid, jasmonic acid, and ethylene synthesis and possibly other response mechanisms in plants (Delledonne 2005; Huang et al 2002; Parani et al 2004)
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