Abstract
BackgroundE-cadherin (CDH1) is a well-known tumor suppressor gene. The loss of CDH1 gene function caused by genetic or epigenetic alterations may consequently result in tumorigenesis. Vitamin C is an essential factor for epigenetic regulation and its great furnished potential in epigenetic therapy in cancer patients. ObjectiveThe present study aims to evaluate the effect of vitamin C on promoter methylation of the CDH1 gene and its possible correlation with the expression of CDH1 in the MCF-7 cell line. MethodsCDH1 expression was studied by reverse transcription-polymerase chain reaction (RT–PCR) analysis in MCF-7 cell line that was treated with different concentrations of vitamin C. TET enzyme activity was measured by ELISA. Promoter methylation of CDH1 was studied by Methylation-Sensitive High-Resolution Melting (MS-HRM) analysis of bisulfite-modified DNA. ResultsOur results showed that vitamin C increase not only TET enzyme activity but also TET gene overexpressed. In the following, cause to CDH1 gene promoter demethylation and upregulation of CDH1 gene in MCF-7 cell line. ConclusionCancer cells may respond to vitamin C induces CDH1 expression by promoter demethylation due to TET enzyme overexpression.
Published Version
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