Abstract
The uptake of Na +-dependent d-glucose by renal brush-border membrane vesicles (BBMV) isolated from streptozotocin-induced diabetic rats was decreased as compared with controls. Since a V max of 4.8 nmol/mg protein per 30 s in diabetic BBMV was significantly decreased as compared with that of controls ( V max = 7.0 nmol/mg protein per 30 s) without changing an apparent affinity for d-glucose, the decrease in the Na +-dependent d-glucose uptake in diabetic rats is likely to be due to the reduction in the number of the transporter. These results are also confirmed by the binding study of [ 3H]phlorizin to diabetic BBMV. When the blood glucose level is lowered in diabetic rats by both the treatment with insulin and starvation, the decreased Na +-dependent d-glucose uptake is returned to control level. These results suggest that Na +-dependent d-glucose reabsorption through the apical membrane in proximal tubular kidney cells is dynamically regulated by the change in blood glucose level.
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