Abstract
The combination of optical manipulation and three-dimensional imaging is a central technique in fields ranging from medicine to physics. Using the objective lens simultaneously for optical trapping and imaging, however, inherently confines the trapping and imaging planes to the same focal plane. Here, we combine remote refocusing microscopy and optical trapping to optically decouple the imaging and trapping planes, achieving aberration-free three-dimensional imaging and simultaneous, decoupled optical trapping without the need for feedback or aberration corrections. We demonstrate our approach by directly imaging the flow field around optically trapped spheres in three dimensions. Due to its compatibility with other imaging and optical manipulation techniques, our approach is relevant to the wide range of fields that combine imaging and optical manipulation, such as physical chemistry, cell biology, and soft matter.
Highlights
The combination of optical manipulation and microscopy is of paramount importance in medicine, biology, chemistry, and physics [1,2,3,4,5,6,7,8]
Aberration corrections can increase this depth to ∼30 μm for optical trapping [20,21], though the low optical efficiency [22] limits its applicability in simultaneous threedimensional (3D) imaging
In this Letter, we present a unique approach that facilitates 3D holographic optical trapping and simultaneous, decoupled imaging using a single microscope objective at the sample
Summary
The combination of optical manipulation and microscopy is of paramount importance in medicine, biology, chemistry, and physics [1,2,3,4,5,6,7,8]. Mechanical or digital wavefront engineering of either the imaging or trapping wavefront can offset its relative axial position [16,17,18], though aberrations in the refocused plane are inevitable.
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