Abstract
The objective of this research was to determine: 1) the decomposition of proteolytic enzymes of earthworm to cottonseed meal; 2) the amino acid content in earthworm, cottonseed meal and the complex which mixed proteolytic enzymes with cottonseed meal. The results showed that the enzymatic activity of proteolytic enzymes in earthworm to cottonseed meal was high. After mixed with proteolytic enzymes, the amino acid content in cottonseed meal increased. Various proportions of essential amino acids is more reasonable. Therefore, using proteolytic enzyme can effectively improve the feed quality of cottonseed meal.
Highlights
Protein content of cottonseed is up to 37~45%, it belongs to soy protein of high quality and its nutritional value is much higher than the cereal proteins (Lawson, 1998)
The objective of this study is to obtain the decomposition of proteolytic enzymes of earthworm to cottonseed meal and the amino acid content in earthworm, cottonseed meal and the complex which mixed proteolytic enzymes with cottonseed meal
Proteolytic enzyme in earthworm’s hydrolyzate can break down cottonseed proteins around the holes, when protein is broken down into amino acids; coomassie blue is eluted under the effect of glacial acetic acid, while other portions without being decomposed into amino acids could not be decolorized
Summary
Protein content of cottonseed is up to 37~45%, it belongs to soy protein of high quality and its nutritional value is much higher than the cereal proteins (Lawson, 1998). Crude protein content in cottonseed meal is 2.89% higher than soybean meal, calcium and phosphorus content were 0.05 and 0.19% higher than soy (Gao et al, 2013). It is rich in all kinds of amino acids, essential amino acids and non-essential amino acids can both be comparable with soybean, so it is high-protein feed resources. The protein content in earthworm is high, accounted for fresh weight of 8 to 10%, accounting for dry weight of 56 to 66% (Khalil et al, 1996). The objective of this study is to obtain the decomposition of proteolytic enzymes of earthworm to cottonseed meal and the amino acid content in earthworm, cottonseed meal and the complex which mixed proteolytic enzymes with cottonseed meal
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